Diana Asks PERM:
Currently, I’ve been doing experimental tests in low field NMR , and I read in the majority of your papers that in order to negate the effect of differences in amplitude due to sample mass, you apply normalization and I´d like to know which normalization method is the best for this spectra?
Dr. Jonathan Bryan from PERM Answers:
Thank you for your question! We cover material like this in our NMR course.
But when we say that we normalize out spectra, we can mean different things:
- If you want to compare results from different machines, then the issue here is that you have amplitudes for each machine. So in order to compare the different machines, you would normalize all your numbers to something like the amplitude per gram of water measured for each machine.
- If you want to compare results of different samples against one another within the same machine, then the issue is something different: here the machine is the same, but the sample sizes are different. In this case we can normalize to the sample masses or sample volumes.
Basically normalization is just something you do in order to be able to compare results properly in different systems. What we normalize to may change for different types of tests, but we pick something that makes sense.
Dr. Jonathan Bryan